Journal: Biochimica et biophysica acta

Article Title: Transgene expression kinetics after transfection with cationic phosphonolipids in hematopoietic non adherent cells.

doi: 10.1016/s0005-2736(98)00002-9

Figure Lengend Snippet: Fig. 2. Comparison of 7 cationic phosphonolipids efficiency in the K562-cell population transfection performed with pCMV bgal plasmid Ž Ž . Ž . Ž . DNA GLB43 RsC18:1, R1sCH , XsI , GLB58 RsC18:1, R1sCH , XsCI , GLB73 RsC14:0, R1sCH , XsI , GLB84 3 3 3 Ž . Ž . Ž . Ž RsC14:0, R1sCH , XsCI , GLB212 RsC18:0, R1sCH , XsI , GLB239 RsC16:0, R1sCH , XsI , GLB416 RsC12:0, 3 3 3 .. Ž . R1sCH , XsI . Three days after transfection, the cells were assayed for b-galactosidase activity with CPRG B and luminescent 3 Ž . I tests. Each point indicates the mean value of the b-galactosidase quantity derived from 4 transfections and the standard deviation from this mean.

Article Snippet: The human hematopoietic non adherent cell line K562 was obtained from American Type Culture Ž .Collection no. ccl 243 ATCC, Rockville, MD, USA Žand was maintained in RPMI-1640 medium Gibco.BRL, Cergy Pontoise, France supplemented with Ž .10% fetal calf serum FCS , 0.2 mM glutamine, 100 Urml of penicillin, 100 U of streptomycin and 1% fungizone.

Techniques: Comparison, Transfection, Plasmid Preparation, Activity Assay, Derivative Assay, Standard Deviation

Journal: Biochimica et biophysica acta

Article Title: Transgene expression kinetics after transfection with cationic phosphonolipids in hematopoietic non adherent cells.

doi: 10.1016/s0005-2736(98)00002-9

Figure Lengend Snippet: Fig. 3. Flow cytometry analysis of the positive cell percentage in a K562-cell population transfected with pCMV bgal plasmid DNA. Ž . Ž . Ž . Ž . Cells were assayed 3 days after lipofection. A Obtained with C14:0 compound GLB 73 and B with C18:1 compound GLB 43 . Ž . Grey curve: negative control K562 cells transfected with a pBR 322 plasmid DNA .

Article Snippet: The human hematopoietic non adherent cell line K562 was obtained from American Type Culture Ž .Collection no. ccl 243 ATCC, Rockville, MD, USA Žand was maintained in RPMI-1640 medium Gibco.BRL, Cergy Pontoise, France supplemented with Ž .10% fetal calf serum FCS , 0.2 mM glutamine, 100 Urml of penicillin, 100 U of streptomycin and 1% fungizone.

Techniques: Flow Cytometry, Transfection, Plasmid Preparation, Negative Control

Journal: Biochimica et biophysica acta

Article Title: Transgene expression kinetics after transfection with cationic phosphonolipids in hematopoietic non adherent cells.

doi: 10.1016/s0005-2736(98)00002-9

Figure Lengend Snippet: Fig. 4. Influence of aliphatic chain length on the transgene kinetic expression, transfections of the K562 cells were performed with various cationic phosphonolipids and pCMV bgal plasmid DNA. The cells were assayed for b-galactosidase activity within 3 and 32 days after transfection. Each data point indicates the mean value of the b-galactosidase quantity derived from four transfections and the standard deviation from this mean.

Article Snippet: The human hematopoietic non adherent cell line K562 was obtained from American Type Culture Ž .Collection no. ccl 243 ATCC, Rockville, MD, USA Žand was maintained in RPMI-1640 medium Gibco.BRL, Cergy Pontoise, France supplemented with Ž .10% fetal calf serum FCS , 0.2 mM glutamine, 100 Urml of penicillin, 100 U of streptomycin and 1% fungizone.

Techniques: Expressing, Transfection, Plasmid Preparation, Activity Assay, Derivative Assay, Standard Deviation

Journal: Biochimica et biophysica acta

Article Title: Transgene expression kinetics after transfection with cationic phosphonolipids in hematopoietic non adherent cells.

doi: 10.1016/s0005-2736(98)00002-9

Figure Lengend Snippet: Fig. 6. Comparison of 6 different DOPE-containing cationic phosphonolipids reagents in transfection assay carried out on a K562-cell population with pCMV bgal plasmid DNA. Three days after transfection, b-galactosidase activity was determined with a luminescent assay. Each data point indicates the mean value of the b-galactosidase quantity derived from four transfections and the standard deviation from this mean.

Article Snippet: The human hematopoietic non adherent cell line K562 was obtained from American Type Culture Ž .Collection no. ccl 243 ATCC, Rockville, MD, USA Žand was maintained in RPMI-1640 medium Gibco.BRL, Cergy Pontoise, France supplemented with Ž .10% fetal calf serum FCS , 0.2 mM glutamine, 100 Urml of penicillin, 100 U of streptomycin and 1% fungizone.

Techniques: Comparison, Transfection, Plasmid Preparation, Activity Assay, Luminescence Assay, Derivative Assay, Standard Deviation

Journal: Biochimica et biophysica acta

Article Title: Transgene expression kinetics after transfection with cationic phosphonolipids in hematopoietic non adherent cells.

doi: 10.1016/s0005-2736(98)00002-9

Figure Lengend Snippet: Fig. 7. Flow cytometry analysis of the positive cell percentage in a K562-cell population transfected with pCMV bgal plasmid DNA. The Ž . cells were assayed 3 days after lipofection. Curves obtained with C14:0 compound GLB73 formulated without DOPE A and with DOPE Ž . Ž . Ž . 1:1 B . Grey curve: negative control K562 cells transfected with a pBR 322 plasmid DNA .

Article Snippet: The human hematopoietic non adherent cell line K562 was obtained from American Type Culture Ž .Collection no. ccl 243 ATCC, Rockville, MD, USA Žand was maintained in RPMI-1640 medium Gibco.BRL, Cergy Pontoise, France supplemented with Ž .10% fetal calf serum FCS , 0.2 mM glutamine, 100 Urml of penicillin, 100 U of streptomycin and 1% fungizone.

Techniques: Flow Cytometry, Transfection, Plasmid Preparation, Negative Control

Journal: Biochimica et biophysica acta

Article Title: Transgene expression kinetics after transfection with cationic phosphonolipids in hematopoietic non adherent cells.

doi: 10.1016/s0005-2736(98)00002-9

Figure Lengend Snippet: Fig. 8. Influence of DOPE on transgene kinetic expression after transfection. K562 cells were transfected with a pCMV bgal plasmid Ž . DNA and assayed for b-galactosidase activity within 3 and 32 days after lipofection with a luminescent assay. A was obtained with Ž . C14:0 compound, GLB 73, B with GLB 84. Both were formulated with and without DOPE. Each data point indicates the mean value of the b-galactosidase quantity derived from four transfections and the standard deviation from this mean.

Article Snippet: The human hematopoietic non adherent cell line K562 was obtained from American Type Culture Ž .Collection no. ccl 243 ATCC, Rockville, MD, USA Žand was maintained in RPMI-1640 medium Gibco.BRL, Cergy Pontoise, France supplemented with Ž .10% fetal calf serum FCS , 0.2 mM glutamine, 100 Urml of penicillin, 100 U of streptomycin and 1% fungizone.

Techniques: Expressing, Transfection, Plasmid Preparation, Activity Assay, Luminescence Assay, Derivative Assay, Standard Deviation

Journal: Biochimica et biophysica acta

Article Title: Transgene expression kinetics after transfection with cationic phosphonolipids in hematopoietic non adherent cells.

doi: 10.1016/s0005-2736(98)00002-9

Figure Lengend Snippet: Fig. 9. Influence of DOPE on transgene kinetic expression after transfection of a K562-cell population with a pCMV bgal plasmid DNA. Ž . b-galactosidase activity was assayed within 3 and 32 days after lipofection with a luminescent assay. A Obtained with C18:1 compound Ž . GLB 43 and B with GLB 58. Both were formulated with and without DOPE. Each data point indicates the mean value of the b-galactosidase quantity derived from four transfections and the standard deviation from this mean.

Article Snippet: The human hematopoietic non adherent cell line K562 was obtained from American Type Culture Ž .Collection no. ccl 243 ATCC, Rockville, MD, USA Žand was maintained in RPMI-1640 medium Gibco.BRL, Cergy Pontoise, France supplemented with Ž .10% fetal calf serum FCS , 0.2 mM glutamine, 100 Urml of penicillin, 100 U of streptomycin and 1% fungizone.

Techniques: Expressing, Transfection, Plasmid Preparation, Activity Assay, Luminescence Assay, Derivative Assay, Standard Deviation

Journal: Biochimica et biophysica acta

Article Title: Transgene expression kinetics after transfection with cationic phosphonolipids in hematopoietic non adherent cells.

doi: 10.1016/s0005-2736(98)00002-9

Figure Lengend Snippet: Fig. 11. Lipoplex B 2.5 h after transfection start: low magnification showing the lipoplex penetration. Bar indicates 1 mm. 11A: Detail: Lipoplex B begins to penetrate in a fold of the cell membrane. Bar indicates 0.2 mm. 11B: Detail: Lipoplex B in the cytoplasm of K562 cell inside a vacuole. Bar indicates 0.2 mm.

Article Snippet: The human hematopoietic non adherent cell line K562 was obtained from American Type Culture Ž .Collection no. ccl 243 ATCC, Rockville, MD, USA Žand was maintained in RPMI-1640 medium Gibco.BRL, Cergy Pontoise, France supplemented with Ž .10% fetal calf serum FCS , 0.2 mM glutamine, 100 Urml of penicillin, 100 U of streptomycin and 1% fungizone.

Techniques: Transfection, Membrane